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Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
BioMed Research International Số , năm 2020 (Tập 2020, trang -)
DOI: 10.1155/2020/7610678
Tài liệu thuộc danh mục: Scopus
BioMed Res. Int.
English
Từ khóa: primer DNA; virus RNA; Betacoronavirus; comparative study; Coronavirus infection; genetics; human; isolation and purification; laboratory technique; multiplex polymerase chain reaction; pandemic; procedures; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA probe; sensitivity and specificity; virology; virus pneumonia; Betacoronavirus; Clinical Laboratory Techniques; Coronavirus Infections; DNA Primers; Humans; Multiplex Polymerase Chain Reaction; Pandemics; Pneumonia, Viral; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA Probes; RNA, Viral; Sensitivity and Specificity
Tóm tắt tiếng anh
Background. There is a shortage of chemical reagents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis and a surge of SARS-CoV-2 cases, especially in limited-resource settings. Therefore, the combination of an optimal assay kit is necessary. Methods. We compared the ability to screen SARS-CoV-2 among three primer-probe sets in two different master mixes, Invitrogen™ SuperScript™ III One-Step RT-PCR and LightCycler Multiplex RNA Virus Master. Results. The assay with TIB-Molbiol, IDT, and Phu Sa sets for LightCycler Multiplex RNA Virus Master or Invitrogen™ SuperScript™ III One-Step RT-PCR showed positive results from a single reaction of triplicate in the three days of 4.8 copies per reaction. R squared and amplification efficiency were 0.97 and ranged from 107 to 108%, respectively. Conclusions. Our findings indicated that TIB-Molbiol, IDT, and Phu Sa primer-probe sets could be beneficial for the laboratory screening of SARS-CoV-2 by RT-qPCR assay of E gene. There is a need to consider the combination of these reagent sets as a new strategy to increase the testing capacity of screening programs for COVID-19. © 2020 Hoang Quoc Cuong et al.
