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An Efficient Method of Observing Diatom Frustules via Digital Holographic Microscopy
Microscopy and Microanalysis Số 6, năm 2022 (Tập 28, trang 2172-2176)
ISSN: 14319276
ISSN: 14319276
DOI:
Tài liệu thuộc danh mục:
Article
English
Tóm tắt tiếng anh
Herein, we propose a convenient method to enable pretreatment of target objects using digital holographic microscopy (DHM). As a test sample, we used diatom frustules (Nitzschia sp.) as the target objects. In the generally used sample preparation method, the frustule suspension is added dropwise onto a glass substrate or into a glass chamber. While our work confirms good observation of purified frustules using the typical sample preparation method, we also demonstrate a new procedure to observe unseparated structures of frustules prepared by baking them on a mica surface. The baked frustules on the mica surface were transferred to a glass chamber with 1% sodium dodecyl sulfate solution. In this manner, the unseparated structures of the diatom frustules were clearly observed. Furthermore, metal-coated frustules prepared by sputtering onto them on a mica surface were also clearly observed using the same procedure. Our method can be applied for the observation of any target object that is pretreated on a solid surface. We expect our proposed method to be a basis for establishing DHM techniques for microscopic observations of biomaterials. The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America.