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Anti-Inflammatory Properties of Longifuran A, a New Benzofuran from the Stems of Amomum longiligulare
Chemistry and Biodiversity Số 12, năm 2021 (Tập 18, trang -)
ISSN: 16121872
ISSN: 16121872
DOI: 10.1002/cbdv.202100518
Tài liệu thuộc danh mục:
Article
English
Từ khóa: Amomum; Animals; Anti-Inflammatory Agents, Non-Steroidal; Benzofurans; Dose-Response Relationship, Drug; Lipopolysaccharides; Mice; Molecular Structure; Nitric Oxide; Plant Stems; RAW 264.7 Cells; 1,7 bis(4 hydroxyphenyl) 1,4,6 heptatrien 3 one; 2,5 dimethoxyphenol; 24 methylcycloartan 3beta ol; 24 methylencycloartan 3beta ol; 4 methoxycinnamic acid; 4,4' dihydroxychalcone; antiinflammatory agent; benzofuran derivative; cardamonin; cyclooxygenase 2; dichloromethane; eudesmic acid; inducible nitric oxide synthase; interleukin 6; lipopolysaccharide; longifuran A; natural product; nitric oxide; phenol derivative; phytochemical; triterpenoid; tumor necrosis factor; unclassified drug; benzofuran derivative; lipopolysaccharide; nitric oxide; nonsteroid antiinflammatory agent; Amomum; Amomum longiligulare; animal cell; antiinflammatory activity; Article; carbon nuclear magnetic resonance; cell viability; controlled study; cytokine release; degree of unsaturation; electrospray mass spectrometry; enzymatic assay; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; IC50; macrophage; nonhuman; nuclear Overhauser effect; phytochemistry; plant stem; protein expression; proton nuclear magnetic resonance; RAW 264.7 cell line; Amomum; animal; biosynthesis; chemical structure; chemistry; dose response; isolation and purification; mouse; plant stem
Tóm tắt tiếng anh
In this study, the following compounds were isolated from the dichloromethane fraction of the stems of Amomum longiligulare and then characterized: a new benzofuran, namely, longifuran A (1); five other phenolic compounds, namely, 4-methoxycinnamic acid (2), 2,5-dimethoxyphenol (3), eudesmic acid (4), 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (5), and 4,4’-dihydroxychalcone (6); and two triterpenoids, namely, 24-methylcycloartan-3β-ol (7) and 24-methylencycloartan-3β-ol (8). They were evaluated in terms of their inhibitory effects on NO production in LPS-stimulated RAW 264.7 macrophages. Results indicated that 1 and 5 exhibited promising inhibitory activities against NO generation with IC50 of 10.47±1.02 μM and 8.51±1.14 μM, respectively. Enzymatic assays demonstrated that they remarkably suppressed the secretion of two pro-inflammatory cytokines (i. e., IL-6 and TNF-α). They also dose-dependently inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2, two important enzymes modulating inflammation. Therefore, 1 and 5 could be targets for the development of new anti-inflammatory therapeutics. © 2021 Wiley-VHCA AG, Zurich, Switzerland
