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Genotyping and macrolide-resistant mutation of Bordetella pertussis in East and South-East Asia

Koide Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan|
Akihiko (7103140857) | Kazunari (7004156769); Saitoh | Nao (38663058200); Kamachi | Noriko (57603950600); Otsuka | Akira (18133727400); Nakajima | Ikuyo (35197138300); Ainai | Chham (24399805500); Takayama | Siphan (57961184400); Samnang | Yong (57195469250); Sovannara Department of Pediatrics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan| Ork (57195474334); Vutthikol Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan| Tran Minh (36096372200); Vichit Research Centre for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan| Do Thu (57581406400); Dien Vaccine-Preventable Diseases and Immunization, World Health Organization, Cambodia| Do Thi Thuy (57615475700); Huong National Immunization Program, Ministry of Health, Cambodia| Phung Thi Bich (57260441500); Nga Surgical Intensive Care Unit, Vietnam National Children's Hospital, Hanoi, Viet Nam| Chuen‑Sheue (57961830200); Thuy Division of General Internal Medicine, Centre for Tropical Diseases, Vietnam National Children's Hospital, Hanoi, Viet Nam| ShuMan (9242497500); Chiang Department of Molecular Biology for Infectious Disease, Vietnam National Children's Hospital, Hanoi, Viet Nam| Kentaro (57205372481); Yao Centre for Diagnostics and Vaccine Development, Centres for Disease Control, Taipei, Taiwan|

Journal of Global Antimicrobial Resistance Số , năm 2022 (Tập 31, trang 263-269)

ISSN: 22137165

ISSN: 22137165

DOI:

Tài liệu thuộc danh mục:

Article

English

Từ khóa: macrolide; RNA 23S; Article; bacterial mutation; bacterium isolation; Bordetella pertussis; Cambodia; Chinese; cladistics; DNA extraction; genotype; genotyping; human; Japan; macrolide resistance; major clinical study; molecular phylogeny; multilocus variable number tandem repeat analysis; real time polymerase chain reaction; sequence database; Taiwan; Taiwanese; variable number of tandem repeat; Viet Nam; whole genome sequencing
Tóm tắt tiếng anh
Objectives: Macrolide-resistant Bordetella pertussis (MRBP) has been emerging and prevailing in mainland China since 2011. In this study, we aimed to investigate the genotype and macrolide resistance of circulating B. pertussis in East and Southeast Asia using genetic analyses. Methods: A total of 302 DNA extracts from clinical specimens and isolates from 2010 to 2020 were analyzed: 145 from Vietnam, 76 from Cambodia, 48 from Taiwan, and 33 from Japan. Genotypes were determined by multilocus variable-number tandem-repeat analysis (MLVA). Macrolide-resistant A2047G mutation in B. pertussis 23S rRNA was investigated using the duplex Cycleave real-time polymerase chain reaction (PCR) assay. Whole-genome sequencing was performed on two MRBP isolates that were identified for the first time in Taiwan. Results: Overall, 286 DNA extracts (95%) generated a complete MLVA genotype and 283 DNA extracts (94%) yielded a complete result for the A2047G mutation analysis. The A2047G mutation was detected in 18 DNA extracts: fourteen from Vietnam, one from Cambodia, two from Taiwan, and one from Japan. Most of them (78%) showed the genotypes MT104 and MT195, which have previously been reported in Chinese MRBP isolates. Further, the Taiwanese MRBP isolates were classified into the MT104 clade of Chinese MRBP isolates. Conclusion: After MRBP emerged and spread in mainland China, it may have spread to East and Southeast Asia in the 2010s. Continued surveillance targeting the A2047G mutation of MRBP is needed to prevent further spread of this emerging pathogen. � 2022

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