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γδTCR+ cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin
Journal of Reproductive Immunology Số 1, năm 2010 (Tập 84, trang 52-56)
ISSN: 1650378
ISSN: 1650378
DOI: 10.1016/j.jri.2009.10.003
Tài liệu thuộc danh mục: Scopus
Article
English
Từ khóa: granulysin; T lymphocyte receptor delta chain; T lymphocyte receptor gamma chain; lymphocyte antigen receptor; amino acid sequence; amino terminal sequence; animal cell; animal tissue; article; carboxy terminal sequence; cell granule; controlled study; endometrium; enzyme activity; female; gamma delta t lymphocyte; gene expression; gene rearrangement; gene sequence; human; human versus animal comparison; mouse; nonhuman; nucleotide sequence; priority journal; protein analysis; protein binding; protein depletion; sequence alignment; sequence homology; sheep; T lymphocyte; T lymphocyte receptor gene; third trimester pregnancy; animal; epithelium; immunology; pregnancy; uterus; Animals; Epithelium; Female; Pregnancy; Receptors, Antigen, T-Cell, gamma-delta; Sheep; T-Lymphocytes; Uterus
Tóm tắt tiếng anh
γδ T cells are a prominent granulated cell population in the ruminant pregnant uterus and both their number and granule size increase dramatically during pregnancy. Anchor-RT-PCR was used to assess TCRδ gene usage by γδ T cells from the uterine epithelium of pregnant sheep. The TCRδ genes obtained exhibited distinct combinatorial and junctional diversity and only two out of nine V-D-J rearrangements sequenced were identical. Furthermore, two of the Vδ elements used are also expressed in peripheral blood, indicating that γδTCR use in sheep epithelia is neither restricted nor site-specific, similar to humans but in contrast to findings in mice. Protein analysis of purified, granulated uterine γδ T cells revealed the presence of large amounts of the antimicrobial peptide, granulysin. The results of the present study indicate that ovine uterine γδTCR+ intraepithelial lymphocytes have the potential to recognise diverse antigens and may have a role in protecting the uterus from infection during pregnancy and parturition. A similar protective role for γδ T cells may exist in the human decidua parietalis. © 2009 Elsevier Ireland Ltd. All rights reserved.
