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Transformation of potato via Agrobacterium coated microparticle bombardment
Biologia Plantarum Số 1, năm 2010 (Tập 54, trang 141-144)
ISSN: 63134
ISSN: 63134
DOI: 10.1007/s10535-010-0022-2
Tài liệu thuộc danh mục: Scopus
Article
English
Từ khóa: Agrobacterium; Agrobacterium tumefaciens; Rhizobium; Solanum tuberosum
Tóm tắt tiếng anh
The transformation of potato (Solanum tuberosum L. cv. Dsire) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm-3. Springer Science+Business Media B.V 2010.