LIÊN KẾT WEBSITE
Protective effect of osmundacetone against neurological cell death caused by oxidative glutamate toxicity
Biomolecules Số 2, năm 2021 (Tập 11, trang 1-13)
ISSN: 2218273X
ISSN: 2218273X
DOI: 10.3390/biom11020328
Tài liệu thuộc danh mục:
Article
English
Từ khóa: Animals; Antioxidants; Apoptosis; Biphenyl Compounds; Calcium; Cell Death; Cell Line; Cell Survival; Chromatin; Embryophyta; Free Radical Scavengers; Glutamic Acid; Heme Oxygenase-1; Hippocampus; HSP70 Heat-Shock Proteins; Humans; MAP Kinase Signaling System; Membrane Proteins; Mice; Neurodegenerative Diseases; Neurons; Neuroprotective Agents; Oxidative Stress; Oxygen; Phosphorylation; Picrates; Plant Extracts; Plants, Medicinal; Reactive Oxygen Species; acetic acid ethyl ester; acetone; acetylcysteine; butanol; calcium; chloroform; dextro glutamic acid; dichlorodihydrofluorescein diacetate; heat shock protein 70; heme oxygenase; heme oxygenase 1; hexane; mitogen activated protein kinase; osmundacetone; propidium iodide; reactive oxygen metabolite; sephadex; streptomycin; stress activated protein kinase; synaptophysin; unclassified drug; 1,1-diphenyl-2-picrylhydrazyl; antioxidant; biphenyl derivative; calcium; glutamic acid; heat shock protein 70; heme oxygenase 1; Hmox1 protein, mouse; membrane protein; neuroprotective agent; oxygen; picric acid; plant extract; reactive oxygen metabolite; scavenger; animal cell; apoptosis; Article; cell viability; cell viability assay; chromatin; chromatin condensation; chromatography; DPPH radical scavenging assay; excitotoxicity; flow cytometry; HT22 cell line; mouse; nerve degeneration; neuronal cell line; neuroprotection; nonhuman; nuclear magnetic resonance imaging; oxidative stress; protein expression; protein phosphorylation; staining; Western blotting; animal; cell death; cell line; cell survival; degenerative disease; Embryophyta; genetics; hippocampus; human; MAPK signaling; medicinal plant; metabolism; nerve cell; oxidative stress; phosphorylation
Tóm tắt tiếng anh
Oxidative stress is one of the main causes of brain cell death in neurological disorders. The use of natural antioxidants to maintain redox homeostasis contributes to alleviating neuro-degeneration. Glutamate is an excitatory neurotransmitter that plays a critical role in many brain functions. However, excessive glutamate release induces excitotoxicity and oxidative stress, leading to programmed cell death. Our study aimed to evaluate the effect of osmundacetone (OAC), isolated from Elsholtzia ciliata (Thunb.) Hylander, against glutamate-induced oxidative toxicity in HT22 hip-pocampal cells. The effect of OAC treatment on excess reactive oxygen species (ROS), intracellular calcium levels, chromatin condensation, apoptosis, and the expression level of oxidative stress-re-lated proteins was evaluated. OAC showed a neuroprotective effect against glutamate toxicity at a concentration of 2 μM. By diminishing the accumulation of ROS, as well as stimulating the expression of heat shock protein 70 (HSP70) and heme oxygenase-1 (HO-1), OAC triggered the self-defense mechanism in neuronal cells. The anti-apoptotic effect of OAC was demonstrated through its inhibition of chromatin condensation, calcium accumulation, and reduction of apoptotic cells. OAC significantly suppressed the phosphorylation of mitogen-activated protein kinases (MAPKs), including c-Jun NH2-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38 kinases. Thus, OAC could be a potential agent for supportive treatment of neurodegenerative diseases. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
