• Chỉ mục bởi
  • Năm xuất bản

Validation of a two-plate microbiological method for screening antibiotic residues in shrimp tissue

Dang P.K. Faculty of Animal Sciences and Aquaculture, Hanoi University of Agriculture, Gialam, Hanoi, Viet Nam|
Scippo M.-L. | Maghuin-Rogister G. | Ton V.D. | Delahaut P. | Pierret G. | Danyi S. Laboratory of Hormonology, CER Groupe, Marloie, Belgium|

Analytica Chimica Acta Số 02-Jan, năm 2010 (Tập 672, trang 30-39)

ISSN: 32670

ISSN: 32670

DOI: 10.1016/j.aca.2010.03.055

Tài liệu thuộc danh mục: Scopus



Từ khóa: Animal-food products; Antibiotic residues; Bacillus Subtilis; Culture conditions; Detection capability; Enrofloxacin; European Commission; Maximum residue limits; Microbiological methods; Microbiological screening; Petri plates; Quinolones; Screening tests; Shrimp tissues; Subtilis; Agriculture; Amides; Aquaculture; Bacteriology; Food products; Liquid chromatography; Mass spectrometry; Sulfur compounds; Antibiotics; acetone; acetonitrile; chlortetracycline; ciprofloxacin; difloxacin; doxycycline; drug residue; enrofloxacin; flumequine; nordifloxacin; oxolinic acid; oxytetracycline; sulfadiazine; sulfadimethoxine; sulfamethoxazole; tetracycline; animal tissue; antibiotic sensitivity; article; Bacillus subtilis; bacterial strain; bacterium culture; controlled study; culture medium; drug determination; drug screening; food contamination; liquid chromatography; mass spectrometry; microbiological examination; minimum inhibitory concentration; nonhuman; pH; priority journal; sensitivity and specificity; shrimp; two plate microbiological method; validation process; Animals; Anti-Bacterial Agents; Bacillus subtilis; Drug Residues; Food Contamination; Microbial Sensitivity Tests; Palaemonidae; Sensitivity and Specificity; Animalia; Bacillus subtilis; Decapoda (Crustacea)
Tóm tắt tiếng anh
Microbiological inhibition screening tests could play an important role to detect residues of antibiotics in the different animal food products, but very few are available for the aquaculture products in general, and for shrimps in particular. A two-plate microbiological method to screen shrimp for residues of the most commonly used antibiotics has been developed and validated according to criteria derived from the European Commission Decision 2002/657/CE. Bacillus subtilis was used as a sensitive strain to target antibiotics. Culture conditions on Petri plates (pH of medium) were selected to enhance the capacity of antibiotic detection. Antibiotic residues were extracted from shrimps using acetonitrile/acetone (70/30, v/v) before application on Petri plates seeded with B. subtilis. The method was validated using spiked blank tissues as well as antibiotic treated shrimps with enrofloxacin and tetracycline, two antibiotics often found to be used in shrimp production. For tetracyclines and (fluoro)quinolones, the detection capability was below the maximum residue limit (MRL), while it was around the MRL for sulfonamides. The specificity of the microbiological screening was 100% in all cases while the sensitivity and accuracy was 100% in almost all cases. The capacity of the method to detect contaminated samples was confirmed on antibiotic treated shrimps, analyzed in parallel with a confirmatory method (Liquid Chromatography coupled to mass spectrometry (LC-MS)). � 2010 Elsevier B.V.

Xem chi tiết